What are the best Assays to measure substances in a 96 well format?

 

1.       LDH Cytotoxicity Assay Kit          C2LD-100             100       £119.00             4°C        For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis. Key Features: Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method: OD500nm. Samples: Cell culture. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

2.       CellQuanti-Blue™ Cell Viability Assay Kits             CQBL-05K          5000     £209.00             4°C        Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. ³H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day. Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: FL530/590nm. Samples: Cell culture. Species: All. Procedure: Assay takes 1-5 hrs, hands-on time 30 min. Kit size: 5000 tests. Detection limit: 100 cells.

3.       CellQuanti-Blue™ Cell Viability Assay Kits             CQBL-10K          10000   £339.00             4°C        Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. ³H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day. Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: FL530/590nm. Samples: Cell culture. Species: All. Procedure: Assay takes 1-5 hrs. Kit size: 10000 tests. Detection limit: 100 cells.

4.       CellQuanti-MTT™ Cell Viability Assay Kits             CQMT-500         500       £209.00             -20°C    Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents. Key Features: Safe. Non-radioactive assay (cf. ³H-thymidine incorporation assay). Sensitive and accurate. As low as 950 cells can be accurately quantified. Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems. Method: OD570nm. Samples: Cell culture. Species: All. Procedure: Assay takes 5 hrs, hands-on time 30 min. Kit size: 500 tests. Detection limit: 950 cells.

5.       Cytotoxicity Control Reagent (Saponin) CTTX-050       (50mg) £109.00             RT         Useful as a cytotoxic control agent in the CQBL-05K and CQMT-500 Cell Viability Assay Kits. IC₅₀ = 0.002 wt%. Shelf life:12 months.

6.       QuantiChrom™ Lactate Dehydrogenase Kit             D2DH-100         100       £349.00             -20°C    For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators. Key Features: High sensitivity and wide linear range. Use 3 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD565nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 U/L.

7.       QuantiChrom™ Nitric Oxide Assay Kit    D2NO-100       100       £329.00             -20°C    For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl₃ reagent, the time required for reduction of nitrate (NO₃^-) to nitrite (NO₂^-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.

8.       QuantiChrom™ Peroxidase Assay Kit     D2PD-100       100       £339.00             -20°C    For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.

9.       QuantiChrom™ Acetylcholinesterase Assay Kit             DACE-100          100       £419.00             -20 °C   For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Key Features: Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD412nm. Samples: Blood, serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 10 U/L.

10.   QuantiChrom™ Acid Phosphatase Assay Kit             DACP-100          100       £169.00             -20 °C   For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.05 to 60 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.05 U/L.

11.   QuantiChrom™ Alcohol dehydrogenase Assay Kit         DADH-100         100       £389.00             -20 °C   For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. plasma, serum, urine, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

12.   QuantiChrom™ α-Glucosidase Assay Kit DAGD-100       100       £469.00             -20 °C   For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.

13.   QuantiChrom™ Alkaline Phosphatase Assay Kit         DALP-250          250       £369.00             -20 °C   For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate. Key Features: High sensitivity and wide linear range. Use 5 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 4 min. Kit size: 250 tests. Detection limit: 2 U/L.

14.   QuantiChrom™ α-Mannosidase Assay Kit             DAMA-100        100       £419.00             4 °C       For quantitative determination of α-mannosidase enzyme activity. Key Features: Fast and sensitive. Linear detection range (10 µL sample): 1 to 250 U/L for a 10 minute reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Biological samples e.g. plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 U/L.

15.   QuantiChrom™ Arginase Assay Kit (100T)             DARG-100         100       £319.00             -20 °C   For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).

16.   QuantiChrom™ ATPase/GTPase Assay Kit             DATG-200          200       £389.00             4 °C       For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.

17.   QuantiChrom™ β-Glucosidase Assay Kit DBGD-100       100       £469.00             -20 °C   For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.

18.   QuantiChrom™ Biotin Assay Kit              DBIO-100       100       £229.00             -20 °C   For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.

19.   QuantiChrom™ Boron Assay Kit DBOR-100             100       £339.00             4 °C       For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.

20.   QuantiChrom™ Carbonyl Assay Kit         DCAR-100       100       £349.00             -20 °C   For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 &microM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD375nm. Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 12 µM.

21.   QuantiChrom™ Chromium Assay Kit             DCRM-250         250       £459.00             -20 °C   For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm).

22.   QuantiFluo™ Caspase-3 Assay Kit           DCS3-100       100       £279.00             -20 °C   For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors. Key Features: Safe. Non-radioactive assay. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL400/490nm. Samples: Cell and tissue lysate. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests.

23.   QuantiFluo™ Cholesterol Uptake Assay Kit             DCUT-100          100       £299.00             -20 °C   For quantitatve determination of cholesterol uptake in cells and screening of modulators on cholesterol uptake. Key Features: Convenient. Treat cells directly in 96-well fluorescent plate. Safe. Non-radioactive assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day Method: FL485/535nm. Samples: Adherent cells. Species: All. Procedure: Assay takes 24-72 hrs, hand-on time 1 hr. Kit size: 100 tests.

24.   QuantiChrom™ Detergent Assay Kit.      DDTR-100       100       £429.00             4 °C       For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.

25.   QuantiChrom™ Free Amino Nitrogen Assay Kit             DFAN-100          100       £259.00             4 °C       For quantitative determination of free amino nitrogen in foods and beverages. Key Features: Fast and sensitive. Linear detection range (5 µL sample): 0.2 to 10 mM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD575nm. Samples: Food and Beverages. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

26.   QuantiChrom™ Fatty Acid Uptake Assay Kit             DFFU-100          100       £429.00             -20 °C   For quantitative determination of long-chain fatty acid uptake in whole cells and evaluation of effects of ligands or drugs on fatty acid transport. Key Features: Safe. Non-radioactive assay. Fast and Sensitive. Homogenous "add-and-read" assay. No wash, lysis, or staining steps needed. Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells. Method: FL 488/523nm. Samples: Adipocytes and other fatty acid transporting cells. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 Tests.

27.   QuantiChrom™ Formaldehyde Assay Kit             DFOR-100          100       £369.00             4 °C       For quantitative and direct determination of formaldehyde concentrations in biological, food and environmental samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL370/470nm. Samples: Biological, food, beverage, environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 1.5 µM (45 ppb).

28.   QuantiChrom™ FRAP Assay Kit  DFRAP-250             250       £389.00             5 °C       For quantitative determination of ferric reduction antioxidant potential (FRAP). Key Features: Sensitive and accurate. Linear detection range: 0.5 to 180 µM Fe³⁺ reduction potential in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Method: OD590nm. Samples: Plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine. Species: All. Procedure: Assay takes 40 min. Kit size: 250 tests. Detection limit: 0.5 µM.

29.   QuantiChrom™ α-L-Fucosidase Assay Kit             DFUC-100          100       £429.00             4 °C       For quantitative determination of α-L-Fucosidase activity in biological samples. Key Features: High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L. Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day. Method: OD405nm. Samples: Plasma, serum, tissue, cell lysate, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 U/L.

30.   QuantiChrom™ Glucose dehydrogenase Assay Kit         DGDH-100         100       £379.00             -20 °C   For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, tissue and culture media). Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

31.   QuantiChrom™ Glutamate dehydrogenase Assay Kit            DGLDH-100       100             £399.00             -20 °C   For quantitative determination of glutamate dehydrogenase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell, tissue, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

32.   QuantiChrom™ Glyoxalase I Assay Kit    DGLO-100       100       £409.00             -20 °C   For quantitative determination of glyoxalase enzyme activity. Key Features: Fast and sensitive. Detection limit of 4 U/L GLO-1 activity. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV). Samples: Enzyme preparations or biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 4 U/L.

33.   QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit        DGPDH-100       100             £419.00             -20 °C   For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

34.   QuantiChrom™ Glutathione S-transferase Assay Kit            DGST-100          100             £329.00             4 °C       For quantitative determination of glutathione S-transferase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 2 to 80 U/L for 10 min reaction at 25°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Biological samples (e.g. cell lysates, tissues, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.

35.   QuantiChrom™Hydroxyproline Assay Kit             DHYP-100          100       £489.00             4 °C       For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.

36.   QuantiChrom™ BCG Albumin Assay Kit  DIAG-250       250       £349.00             -20 °C   For quantitative determination of albumin and evaluation of drug effects on albumin metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Detection range 0.01 - 5 g/dL (1.5 - 750 µM) albumin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. No interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD620nm (BCG). Samples: Serum, plasma, urine, biological preparations. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.01 g/dL.

37.   QuantiChrom™ BCP Albumin Assay Kit  DIAP-250       250       £369.00             -20 °C   For quantitative determination of albumin and evaluation of drug effects on albumin metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Detection range 0.3 - 5 g/dL (45 - 750 µM) albumin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD610nm (BCP). Samples: Serum, plasma, urine, biological preparations. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.3 g/dL.

38.   QuantiChrom™ Bilirubin Assay Kit          DIBR-180       180       £359.00             4 °C       For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL.

39.   QuantiChrom™ Calcium Assay Kit           DICA-500       500       £389.00             4°C        For quantitative determination of calcium ion Ca²⁺ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).

40.   QuantiChrom™ Chloride Assay Kit          DICL-250       250       £329.00             4°C        For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl^- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM).

41.   QuantiChrom™ Creatinine Assay Kit      DICT-500       500       £399.00             -20 °C   For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).

42.   QuantiChrom™ Copper Assay Kit            DICU-250       250       £479.00             4 °C       For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM).

43.   QuantiChrom™ Indican Assay Kit            DIDC-01K       1000     £419.00             -20 °C   For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 0.2 mg/dL.

44.   QuantiChrom™ Indican Assay Kit            DIDC-100       100       £309.00             -20 °C   For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.2 mg/dL.

45.   QuantiChrom™ Isocitrate Dehydrogenase Assay Kit            DIDH-100           100             £419.00             -20 °C   For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 100 U/L for 30 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Plasma, serum,tissue and culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

46.   QuantiChrom™ Ethanol Assay Kit           DIET-500       500       £489.00             4°C        For quantitative determination of ethanol and alcohol metabolism. Key Features: Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay. Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD580nm (Chemical). Samples: Alcoholic beverages etc. Species: All. Procedure: Assay takes 10 min. Kit size: 500 tests. Detection limit: 0.04%.

47.   QuantiChrom™ Iron Assay Kit    DIFE-250             250       £419.00             -20 °C   For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism. Key Features: Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Biological (e.g. serum) and environmental samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 27 µg/dL (4.8 µM).

48.   QuantiChrom™ Glucose Assay Kit           DIGL-100       100       £319.00             -20 °C   For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples. Method: OD630nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.7 mg/dL (39 µM).

49.   QuantiChrom™ Glutathione Assay Kit    DIGT-250       250       £429.00             4 °C       For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on glutathione metabolism. Key Features: Sensitive and accurate. Linear detection range 0.4 - 100 µM in 96-well plate. Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 12 µg/dL (0.4 µM).

50.   QuantiChrom™ Hemoglobin Assay Kit   DIHB-250       250       £369.00             4 °C       For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Key Features: Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.9 mg/dL.

51.   QuantiChrom™ Heme Assay Kit DIHM-250             250       £379.00             4 °C       For quantitative determination of heme and evaluation of drug effects on heme metabolism. Key Features: Sensitive and accurate. Linear detection range 0.6 - 125 µM heme in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents are non-toxic. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.6 µM.

52.   QuantiChrom™ Hyaluronidase Inhibitor Screening Assay Kit       DIHY-100           100             £489.00             -20 °C   For evaluation and high-throughput screen (HTS) of hyaluronidase modulators. Key Features: Rapid and reliable. Entire assay can be completed in 30 min. Simple and Convenient. Simple procedure with an enzymatic reaction and addition of stop reagent. No wash or reagent transfer steps involved. Robust and amenable to HTS. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Turbidimetric (OD 600nm). Samples: Test Compounds, Hyaluronidase Inhibitors. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

53.   QuantiChrom™ Magnesium Assay Kit    DIMG-250       250       £409.00             4 °C       For quantitative determination of Mg²⁺ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).

54.   QuantiChrom™ Indole Assay Kit             DIND-100       100       £199.00             4 °C       For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM.

55.   QuantiChrom™ Peroxide Assay Kit         DIOX-250       250       £349.00             4 °C       For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism. Key Features: Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H₂O₂ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD585nm. Samples: Serum, citrate-plasma, urine, cell lysate, culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 µM.

56.   QuantiChrom™ Phosphate Assay Kit      DIPI-500       500       £349.00             4 °C       For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM).

57.   QuantiChrom™ Uric Acid Assay Kit         DIUA-250       250       £439.00             -20 °C   For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).

58.   QuantiChrom™ Urea Assay Kit (100T)    DIUR-100       100       £199.00             -20 °C   For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

59.   QuantiChrom™ Zinc Assay Kit    DIZN-250             250       £489.00             4 °C       For quantitative determination of zinc ion Zn²⁺ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).

60.   QuantiChrom™ Lipase Assay Kit             DLPS-100       100       £469.00             4 °C       For quantitative determination of lipase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Method: OD412nm. Samples: Serum, plasma, saliva, urine etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

61.   QuantiChrom™ β-N-Acetylglucosaminidase Assay Kit            DNAG-100         100             £429.00             -20 °C   For quantitatve determination of β-N-Acetylglucosaminidase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Urine, serum, plasma, cell lysate, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

62.   QuantiFluo™ Ammonia Assay Kit            DNH3-200       200       £189.00             -20 °C   For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.

63.   QuantiChrom™ Protein Creatinine Ratio Assay Kit         DPCR-100          100       £599.00             -20 °C   For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). Key Features: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 - 150 mg/dL Creatinine. Fast and convenient. No sample pre-treatment is needed. Simple 10- minute "add-incubate-read" procedure. High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Method: OD600 and OD530nm. Samples: Urine samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.

64.   QuantiChrom™ Pectinase Assay Kit        DPEC-100       100       £299.00             -20 °C   For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.

65.   QuantiChrom™ Phytase Assay Kit           DPHT-100       100       £399.00             4 °C       A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

66.   QuantiChrom™ Salicylate Assay Kit             DSALY-100         100       £439.00             2°C to RT           For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests.

67.   QuantiChrom™ Sorbitol Dehydrogenase Assay Kit         DSDH-100          100       £459.00             -20 °C   For quantitative determination of sorbitol dehydrogenase enzyme activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples e.g. plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

68.   QuantiChrom™ Sulfate Assay Kit            DSFT-200       200       £379.00             4 °C       For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. Key Features: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Method: OD600nm. Samples: Serum, urine, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 200 tests. Detection limit: 20 µM.

69.   QuantiChrom™ Sialic Acid Assay Kit       DSLA-100       100       £489.00             -20 °C   For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid. Key Features: Sensitive and accurate. Use as little as 60 µL samples. Linear detection range in 96-well plate: 5 to 1000 µM sialic acid for colorimetric assays and 0.5 to 100 µM for fluorimetric assays. Method: OD549nm, or FL555/585nm. Samples: Serum, plasma, saliva, milk etc. Species: All. Procedure: Assay takes 2.5 hrs. Kit size: 100 tests. Detection limit: OD, FL: 5, 0.5 µM.

70.   Saccharide Removal Kit DSRK-500          500             £229.00             RT         Removal of interfering saccharides (e.g. glucose, sucrose) from samples such as culture media. Key Features: Convenient and high-throughput. The procedure involves addition of three reagents sequentially, incubation for 15 min, centrifugation for 5 min, and transfer of the supernatant. Samples: Culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 500 treatments.

71.   QuantiChrom™ Antioxidant Assay Kit    DTAC-100       100       £329.00             -20 °C   For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range from 1.5 to 1000 µM Trolox equivalents. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD570nm. Samples: Serum, plasma, urine, saliva, food and beverage etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1.5 µM.

72.   QuantiChrom™ TBARS Assay Kit             DTBA-100       100       £249.00             -20 °C   For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. Key Features: Sensitive and accurate. Linear detection range: colorimetric assay 1 - 30 µM, fluorometric assay 0.1 - 1.5 µM MDA. Method: OD535nm, or FL530/550nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 0.1 µM.

73.   QuantiChrom™ HRP Detection Reagent             DTMB-200         200       £159.00             4 °C       A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. Key Features: Highly sensitive. Detects as low as 7.6x10-8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA. Improved stability and assay robustness. Method: OD450nm. Samples: peroxidases and HRP in ELISA. Species: All. Procedure: Assay takes Approx. 30 min. Kit size: 200 Tests. Detection limit: 76 nU/well, or 7.7 pg/well.

74.   QuantiFluo™ Urokinase Inhibitor Screening Assay Kit            DUKI-100           100             £389.00             -20 °C   For evaluation of drugs and screening potential inhibitors of urokinase. Key Features: Safe. Non-radioactive assay. Fast. Assay is completed within a 30 minute reaction time. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to assay thousands of samples per day. Method: FL 380/450 nm; Samples: Purified protein and protein extracts; Species: All. Procedure: assay takes 30min; Size: 100 tests. Detection Limit: 0.04 U/L.

75.   QuantiFluo™ Urokinase Assay Kit           DUKN-100       100       £389.00             -20 °C   For quantitative determination of urokinase activity determination in biological samples. Key Features: Safe. Non-radioactive assay. Fast. Assay is completed within a 15 minute reaction time. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to assay thousands of samples per day. Method: FL380/450 nm; Samples: Biological Samples (e.g. Urine and serum). Species: All. Procedure: assay takes 15min. Size: 100 tests. Detection Limit: 0.04 U/L.

76.   QuantiChrom™ Urea Assay Kit II             DUR2-100       100       £189.00             -20 °C   For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

77.   QuantiChrom™ Urease Assay Kit            DURE-100       100       £429.00             4 °C       For quantitative determination of urease activity and evaluation/screen for urease inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD670nm. Samples: Biological, environment etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.003 U/L.

78.   QuantiChrom™ Whole Blood Hb Kit             DWHB-250        250       £409.00             4 °C       For quantitative determination of hemoglobin (Hb) in whole blood. Key Features: Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvette. High sensitivity. As low as 5 µL sample is used. Ideal when samples are limited such as mice blood samples. Method: OD570nm. Samples: Whole blood samples. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.2 g/dL.

Enzychrom kits for measuring constituents in cell supernatans

 

1.       EnzyChrom™ ADP Assay Kit       E2ADP-100             100       £429.00             -20 °C   For quantitative determination of ADP determination in cells and other biological samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/590nm. Samples: Cells and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.

2.       EnzyChrom™ AF Cholesterol Assay Kit   E2CH-100       100       £439.00             -20 °C   For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 10 mg/dL cholesterol for colorimetric assays and 0.02 to 2 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.

3.       EnzyChrom™ Creatinine Assay Kit          E2CT-100       100       £399.00             -20 °C   For quantitative determination of creatinine and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction.  It is 3- and 53-fold more sensitive than the traditional Jaffe method (e.g. DICT-500), especially useful for small samples or where high sensitivity is required. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm; FL530/585nm. Samples: Urine, serum, plasma, and other biological preparations. Species: All. Procedure: 60 min. Size: 100 tests. Detection Limit: Colorimetric assay: 4.8 µM or 0.054 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL.

4.       EnzyChrom™ Glycogen Assay Kit            E2GN-100       100       £439.00             -20 °C   For quantitative determination of glycogen and evaluation of drug effects on glycogen metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL glycogen for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µg/mL.

5.       EnzyChrom™ AF HDL and LDL/VLDL Assay Kit             E2HL-100           100       £479.00             -20 °C   For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Method: OD570nm, or FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.

6.       EnzyChrom™ NAD/NADH Assay Kit        E2ND-100       100       £479.00             -20 °C   For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µM.

7.       EnzyChrom™ NADP/NADPH Assay Kit    E2NP-100       100       £479.00             -20 °C   For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts. Key Features: Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Colorimetric Determination at 565 nm. Samples: Cells, Tissue. Species: human, mouse, rat and more. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.

8.       EnzyChrom™ Starch Assay Kit    E2ST-100             100       £419.00             -20 °C   For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

9.       EnzyFluo™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit          E4EBP-100             100       £509.00             -20 °C   For determination of 4EBP1(S65) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human (S65), mouse/rat (S64). Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

10.   EnzyChrom™ Acetylcholine Assay Kit     EACL-100       100       £439.00             -20 °C   For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 10 to 200 µM, fluorimetric assay 0.4 to 10 µM acetylcholine. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 10, 0.4 µM.

11.   EnzyChrom™ Aconitase Assay Kit           EACO-100       100       £379.00             -20 °C   For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

12.   EnzyChrom™ Acetaldehyde Assay Kit    EACT-100       100       £329.00             -20 °C   For quantitatve determination of acetaldehyde enzyme activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20µL sample): 2µM to 2 mM acetaldehyde in 96-well plate assay. High-throughput.The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. Convenient.Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µM.

13.   EnzyChrom™ Aldehyde Dehydrogenase Assay Kit         EADH-100          100       £399.00             -20 °C   For quantitative determination of aldehyde dehydrogenase activity in biological samples (e.g serum, cell lysate, etc). Key Features: Sensitive and accurate. Linear detection range 0.2 - 25 U/L ALDH in a 96-well plate assay. Convenient. The assay involves addition of a single working reagent and can be completed in under an hour. Robust and amenable to HTS. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: biological samples (serum, cell lysate, etc). Species: All. Procedure: 30min. Size: 100 tests.

14.   EnzyLight™ ADP Assay Kit          EADP-100             100       £359.00             -20 °C   For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 µM.

15.   EnzyFluo™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit          EAKT1-100             100       £509.00             -20 °C   For determination of AKT1(S473) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

16.   EnzyChrom™ L-Alanine Assay Kit            EALA-100       100       £419.00             -20 °C   For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 µM alanine for colorimetric assays and 0.4 to 20 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.4 µM.

17.   EnzyChrom™ Alanine Transaminase Assay Kit             EALT-100           100       £349.00             -20 °C   For quantitative determination of alanine transaminase enzyme activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.

18.   EnzyFluo™ AMPK Phosphorylation Assay Kit             EAMPK-100       100       £559.00             -20 °C   For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

19.   EnzyChrom™ Adipogenesis Assay Kit     EAPG-200       100       £429.00             -20 °C   For sensitive quantitative determination of adipogenesis and high-throughput screening of adipogenesis modulators. Key Features: Sensitive and accurate. Use as little as 40 µL samples. Linear detection range in 96-well plate: 0.16 to 5 nmoles for colorimetric assays and 0.075 to 0.5 nmoles for fluorimetric assays. Fast and convenient. The addition of a single working reagent and 30-min incubation procedure combines sample extraction, hydrolysis and color reaction. The whole procedure is performed at room temperature with NO incubator or heating needed. Robust and amenable to HTS. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: biological samples (cells, tissues, etc). Species: All. Procedure: 30min. Size: 200 tests.

20.   EnzyChrom™ Adipolysis Assay Kit           EAPL-200       200       £399.00             -20 °C   For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.

21.   EnzyChrom™ Ascorbic Acid Assay Kit     EASC-100       100       £429.00             -20 °C   For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: OD, FL: 6, 1 µM.

22.   EnzyChrom™ Aspartate Assay Kit           EASP-100       100       £419.00             -20 °C   For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism. Key Features: Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colorimetric assays and 1 to 50 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue, culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 1 µM.

23.   EnzyChrom™ Aspartate Transaminase Assay Kit         EASTR-100         100       £399.00             -20 °C   For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.

24.   EnzyLight™ ATP Assay Kit           EATP-100             100       £349.00             -20 °C   For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.

25.   EnzyChrom™ Glucose Assay Kit EBGL-100             100       £379.00             -20 °C   For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 5 to 300 µM (90 µg/dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 µM for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 µM.

26.   EnzyChrom™ α-Amylase Assay Kit          ECAM-100       100       £459.00             -20 °C   For quantitative determination of α-amylase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm. Method: OD585nm. Samples: Blood, saliva, urine, agriculture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.3 U/L.

27.   EnzyChrom™ Catalase Assay Kit ECAT-100             100       £339.00             -20 °C   For quantitative determination of catalase activity and evaluation of drug effects on catalase activity. Key Features: Sensitive and accurate. Use 10 µL sample. Linear detection range 0.2 to 5 U/L catalase activity. Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

28.   EnzyChrom™ Cholesterol Assay Kit        ECCH-100       100       £439.00             -20 °C   For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Key Features: Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 mg/dL.

29.   EnzyChrom™ Ethanol Assay Kit ECET-100             100       £419.00             -20 °C   For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. Key Features: Sensitive and accurate. Detection limit 0.0008 vol % (140 µM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay. Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm (Enzymatic). Samples: Serum, plasma, urine, saliva samples etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.0008%.

30.   EnzyChrom™ Glycolysis Assay Kit           ECGL-100       100       £379.00             -20 °C   For quantitative determination of L-Lactate and screening for glycolysis modulators. Key Features: Fast and sensitive. Use of 5 µL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell media. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

31.   EnzyChrom™ Glutathione Reductase Kit             ECGR-100          100       £319.00             -20 °C   For quantitative determination of glutathione reductase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

32.   EnzyChrom™ Choline Assay Kit  ECHO-100             100       £439.00             -20 °C   For quantitative determination of choline and evaluation of drug effects on choline metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, cells etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 µM.

33.   EnzyChrom™ Isocitrate Assay Kit            ECIC-100       100       £409.00             -20 °C   For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

34.   EnzyChrom™ Citrate Assay Kit   ECIT-100             100       £429.00             -20 °C   For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism. Key Features: Fast and sensitive. Linear detection range: 4 to 400 µM citrate for colorimetric assays and 0.5 to 40 µM for fluorimetric assays. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, agriculture etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.5 µM.

35.   EnzyChrom™ Lactate Assay Kit  ECLC-100             100       £439.00             -20 °C   For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

36.   EnzyChrom™ Coenzyme A Assay Kit       ECOA-100       100       £439.00             -20 °C   For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism. Key Features: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 - 1000 µM, fluorimetric assay 3 - 100 µM CoA. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3 µM.

37.   EnzyFluo™ Collagen Assay Kit    ECOL-100             100       £439.00             -20 °C   For quantitatve determination of collagen in biological and cosmetic products. Key Features: Safe. Non-radioactive assay. Fast and sensitive. Linear detection range (20µL sample): 2 µg/mL to 50µg/mLcollagen in 96-well plate assay. Convenient and high-throughput."Add-mix-read" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: FL375/465nm. Samples: Biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution). Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

38.   EnzyChrom™ Creatine Kinase Assay Kit ECPK-100       100       £519.00             -20 °C   For quantitative determination of creatine kinase (CK) activity and evaluation of drug effects on CK activity. Key Features: Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 U/L.

39.   EnzyChrom™ Creatine Assay Kit             ECRT-100       100       £429.00             -20 °C   For quantitative determination of creatine and evaluation of drug effects on creatine metabolism. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes. Method: OD570nm, or FL530/590nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 4 µM.

40.   EnzyLight™ Cytotoxicity Assay Kit           ECTX-100       100       £289.00             -20 °C   For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.

41.   EnzyChrom™ D-Amino Acid Assay Kit     EDAA-100       100       £489.00             -20 °C   For quantitative determination of D-Amino Acids in biological and other samples. Key Features: Fast and sensitive. Linear detection range: 0.86 to 500 µM (colorimetric assay) and 0.18 to 50 µM (fluorimetric assay) for 60 min reaction. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: Tissue, milk, and other biological samples. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.86 µM (colorimetric assay), 0.18 µM (fluorimetric assay).

42.   EnzyChrom™ D-Lactate Assay Kit            EDLC-100       100       £429.00             -20 °C   For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

43.   EnzyFluo™ ERK Phosphorylation Assay Kit             EERK-100           100       £569.00             -20 °C   For quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-based ELISA (FL360/450nm, 530/585nm). Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

44.   EnzyFluo™ NADP/NADPH Assay Kit             EF2NP-100        100       £479.00             -20 °C   For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (<i>This product is a replacement for the discontinued product EFNP-100</i>). Key Features: Sensitive and accurate. Detection limit of 0.01 &micro;M and linearity up to 1 &micro;M NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 &micro;M.

45.   EnzyFluo™ Acetaldehyde Assay Kit         EFAC-100       100       £319.00             -20 °C   For quantitatve determination of acetaldehyde and evaluations of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (50&micro;L sample): 0.5 &micro;M to 60 &micro;M acetaldehyde in 96-well plate assay. High-throughput. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. Convenient. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 &micro;M.

46.   EnzyFluo™ Bile Acid Assay Kit    EFBA-100             100       £499.00             -20 °C   For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 - 150 &micro;M bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 &micro;M.

47.   EnzyFluo™ Custom Cell-Based ELISA Kit             EFC1M-100       100       £359.00             -20 °C   A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary mouse antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

48.   EnzyFluo™ Custom Cell-Based ELISA Kit             EFC2R-100         100       £359.00             -20 °C   A reagent kit that allows our customers to develop their own cell-based ELISA. Determination of relative protein content and signal pathway (e.g. phosphorylation status) in whole cells. Evaluation of target modulation by drugs, activators, inhibitors, siRNA etc. All that is required is a primary rabbit antibody (Ab1) for the specific target of interest. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Target protein is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, rat and more, depending on choice of primary antibody. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

49.   EnzyFluo™ D-Lactate Assay Kit  EFDLC-100             100       £439.00             -20 °C   For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 &micro;M and linearity up to 50 &micro;M D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 &micro;M.

50.   EnzyChrom™ Free Fatty Acid Assay Kit  EFFA-100       100       £449.00             -20 °C   For quantitative determination of fatty acid and evaluation of drug effects on its metabolism. Key Features: Sensitive. Use 10 &micro;L samples. Linear detection range: colorimetric assay 7 - 1000 &micro;M, fluorimetric assay 7 - 100 &micro;M fatty acid. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 7 &micro;M.

EnzyFluo to measure Cellular Fluids

 

1.       EnzyFluo™ Glucose Uptake Assay Kit     EFGU-100       100       £669.00             -20 °C   For quantitative determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport. Key Features: Safe. No radioactive material is used. Sensitive and Accurate. Detection limit of 0.1 &micro;M and linearity up to 5 &micro;M 2-DG6P. Simple and Convenient. Can be automated as a medium throughput assay for glucose transport in cells. Method: FL530/585nm. Samples: Cell culture. Species: Mammalian. Procedure: Assay takes Approximately 2 hrs. Kit size: 100 tests. Detection limit: 0.1 &micro;M.

2.       EnzyFluo™ Isocitrate Assay Kit   EFIC-100             100       £399.00             -20 °C   For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 0.6 to 500 &micro;M for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585 nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.6 &micro;M.

3.       EnzyFluo™ L-Lactate Assay Kit   EFLLC-100             100       £439.00             -20 °C   For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 &micro;M and linearity up to 50 &micro;M L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 &micro;M.

4.       EnzyChrom™ Fumarase Assay Kit           EFMR-100       100       £549.00             -20 °C   For quantitative determination of fumarase enzyme activity. Key Features: Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37&deg;C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

5.       EnzyFluo™ NAD/NADH Assay Kit            EFND-100       100       £459.00             -20 °C   For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.02 &micro;M and linearity up to 1 &micro;M NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: F530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 &micro;M.

6.       EnzyChrom™ Formate Assay Kit EFOR-100             100       £459.00             -20 °C   For quantitative determination of formate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 &micro;L sample. Linear detection range 0.050 to 5 mM Formate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Urine, Serum etc. Species: All. Procedure: Assay takes approximately 60 min. Kit size: 100 tests. Detection limit: 50 &micro;M.

7.       EnzyChrom™ Fumarate Assay Kit           EFUM-100       100       £419.00             -20 °C   For quantitative determination of fumarate and drug effects on its metabolism. Key Features: Fast and sensitive. Use of 20 &micro;L sample. Linear detection range 0.005 to 2 mM fumarate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 &micro;M.

8.       EnzyChrom™ Glucose-6-Phosphate Assay Kit             EG6P-100          100       £439.00             -20 °C   For quantitative determination of glucose-6-phosphate in biological samples. Key Features: Fast and sensitive. Linear detection range: 10 to 1000 &micro;M G6P. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 &micro;M.

9.       EnzyChrom™ Galactose Assay Kit           EGAL-100       100       £439.00             -20 °C   For quantitative determination of galactose and evaluation of drug effects on its metabolism. Key Features: Use as little as 20 &micro;L samples. Linear detection range in 96-well plate: 10 to 1000 &micro;M galactose for colorimetric assays and 10 to 100 &micro;M for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 &micro;M.

10.   EnzyChrom™ Glucose Assay Kit II           EGL2-100       100       £389.00             -20 °C   For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.1 to 3 mM (1.8 mg/dL to 54 mg/dL) glucose for colorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.1 mM.

11.   EnzyChrom™ Glucose Assay Kit III          EGL3-100       100       £389.00             -20 °C   For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 &micro;L samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.03 mM.

12.   EnzyChrom™ Glutamine Assay Kit          EGLN-100       100       £489.00             -20 °C   For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism. Key Features: Sensitive and accurate. Use 20 &micro;L sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37&deg;C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 23 &micro;M.

13.   EnzyChrom™ Glutamate Assay Kit          EGLT-100       100       £429.00             -20 °C   For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 &micro;M, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37&deg;C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 &micro;M.

14.   EnzyChrom™ Glycerol Assay Kit EGLY-200             200       £419.00             -20 °C   For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate: 10 to 1000 &micro;M (92 &micro;g/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 &micro;M for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological, food, beverage etc. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 2 &micro;M.

15.   EnzyChrom™ Glucose Oxidase Assay Kit             EGOX-100          100       £389.00             -20 °C   For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 &micro;L samples. Linear detection range in 96-well plate for 20 minute incubation at 25&deg;C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).

16.   EnzyChrom™ Glutahione Peroxidase Assay Kit             EGPX-100          100       £339.00             -20 °C   For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity. Key Features: Sensitive and accurate. Use 10 &micro;L sample. Linear detection range 40 to 800 U/L GPX activity. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

17.   EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit          EGSK3A-100             100       £509.00             -20 °C   For determination of GSK3A(S21) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat, mouse, and canine. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

18.   EnzyFluo™ Human/Mouse GSK3B(S9) Phosphorylation ELISA Kit          EGSK3B-100             100       £509.00             -20 °C   For determination of GSK3B(S9) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

Bioassay measurement kits

 

1.       EnzyChrom™ GSH/GSSG Assay Kit          EGTT-100       100       £349.00             -20 °C   For quantitative determination of reduced/oxidized glutathione (GSH/GSSG) and evaluation of drug effects on their metabolism. Key Features: Sensitive and accurate. Linear detection range 0.01-3 &micro;M GSH equivalents with a detection limit of 10 nM GSH equivalents. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 nM.

2.       EGTT-Scavenger for GSH/GSSG Assays (EGTT-100)      EGTT-SCVG        100       £87.00  RT             EGTT-Scavenger (cat# EGTT-SCVG) is a chemical reagent used for sample treatment with the GSH/GSSG Assay Kit (cat#: EGTT-100). Necessary for cell lysate samples used in the EGTT-100 assay kit. Key Features: Ready-to-use 500µL solution, sufficient for 100 tests with EGTT-100 assay kit.

3.       EnzyChrom™ HDL and LDL/VLDL Assay Kit             EHDL-100          100       £509.00             -20 °C   For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Requires only 20 &micro;L serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37&deg;C heater is needed. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 5 mg/dL.

4.       EnzyChrom™ Histamine Assay Kit           EHIS-100       100       £449.00             -20 °C   For quantitative determination of histamine in food, beverage and agricultural products. Key Features: Fast and sensitive. Use of 20 &micro;L sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2.5 &micro;M.

5.       QuantiChrom™ Aldehyde Dehydrogenase Inhibitor Screening Kit  EIAL-100            100             £318.00             -20 °C   For evaluation and high-throughput screen (HTS) of aldehyde dehydrogenase modulators. Key Features: Rapid and reliable. Can be completed in under an hour. Homogenous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Aldehyde dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

6.       EnzyChrom™ Monoamine Oxidase Inhibitor Screening Kit    EIMO-100          100             £399.00             -20 °C   For evaluation and high-throughput screen (HTS) of monoamine oxidase modulators. Key Features: Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Monoamine Oxidase. Species: All. Procedure: Assay takes 35 min. Kit size: 100 tests.

7.       EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit     EINO-100           100             £459.00             -20 °C   For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators. Key Features: High-throughput. Homogenous &ldquo;mix-incubate-measure&rdquo; type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37&deg;C. Method: OD540nm. Samples: Nitric Oxide Synthase. Species: All. Procedure: Assay takes 2-3 hours. Kit size: 100 tests.

8.       EnzyChrom™ Invertase Assay Kit            EIVT-100       100       £479.00             -20 °C   For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.

9.       EnzyChrom™ Ketone Body Assay Kit      EKBD-100       100       £479.00             -20 °C   For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Uses 10 &micro;L sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day. Method: OD340nm. Samples: Serum, plasma, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.12 mM.

10.   EnzyChrom™ Kinase Assay Kit   EKIN-400             400       £299.00             -20 °C   Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 &micro;M ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.

11.   EnzyChrom™ L-Amino Acid Kit   ELAA-100             100       £419.00             -20 °C   For quantitative determination of L-Amino Acids in biological and other samples. Key Features: Fast and sensitive. Linear detection range: 3.3 to 500 &micro;M (colorimetric assay) and 0.13 to 50 &micro;M (fluorimetric assay) for 60 min reaction. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: Serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3.3 &micro;M (colorimetric assay), 0.13 &micro;M (fluorimetric assay).

12.   EnzyChrom™ Lactose Assay Kit  ELAC-100             100       £429.00             -20 °C   For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 &micro;L samples. Linear detection range in 96-well plate: 17 to 2000 &micro;M lactose for colorimetric assays and 6 to 100 &micro;M for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 &micro;M.

13.   EnzyLight™ ADP/ATP Ratio Assay Kit      ELDT-100       100       £389.00             -20 °C   For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells and screen for modulators. Key Features: Safe. Non-radioactive assay. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

14.   EnzyFluo™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit          ELKB1-100             100       £509.00             -20 °C   For determination of LKB1(S431) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, and rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

15.   EnzyChrom™ Malate Assay Kit  EMAL-100             100       £419.00             -20 °C   For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 &micro;L sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.

16.   EnzyChrom™ Monoamine Oxidase Assay Kit             EMAO-100         100       £409.00             -20 °C   For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: MAO enzyme preparations. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

17.   EnzyChrom™ Malate Dehydrogenase Assay Kit         EMDH-100         100       £459.00             -20 °C   For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 0.5 to 65 U/L for 20 min reaction at 37&deg;C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

18.   EnzyChrom™ Maltose Assay Kit EMLT-100             100       £369.00             -20 °C   For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 &micro;L sample. Linear detection range 2 to 500 &micro;M maltose for colorimetric assays and 1 to 50 &micro;M for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 &micro;M.

19.   EnzyChrom™ D-Mannitol Assay Kit        EMNT-100       100       £479.00             -20 °C   For quantitative determination of D-mannitol in biological, food, beverage and agriculture samples. Key Features: Fast and sensitive. Use of 20 &micro;L sample. Linear detection range 0.007 to 3 mM mannitol in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37&degC heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Biological, food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 7 &micro;M.

20.   EnzyFluoTM Myeloperoxidase Assay Kit              EMPO-100         100       £429.00             -20 °C   For quantitative determination of myeloperoxidase enzyme activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 0.0025 to 2 U/L for 10 min reaction at 25&deg;C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Cell lysates, tissues, etc. Species: All. Procedure: Assay takes approximately 30 min. Kit size: 100 tests. Detection limit: 0.0025 U/L.

21.   EnzyChrom™ Neuraminidase Assay Kit  ENEU-100       100       £529.00             -20 °C   For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor. Key Features: Sensitive and accurate. Linear detection range at 37&deg;C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays. Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.01U/L.

22.   EnzyFluo™ NFkB Phosphorylation Assay Kit             ENFKB-100        100       £559.00             -20 °C   For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

23.   EnzyChrom™ Ammonia Assay Kit           ENH3-100       100       £359.00             -20 °C   For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: High sensitivity and wide linear range. Use 20 &micro;L sample. Linear detection range 24 to 1000 &micro;M ammonia. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes. Method: OD340nm, or FL360/450nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 24 &micro;M.

24.   EnzyChrom™ Nitric Oxide Synthase Assay Kit             ENOS-100          100       £449.00             -20 °C   For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor. Key Features: Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate. Rapid and reliable. Can be completed in 40 min if reduction of NO<SUB>3</SUB><SUP>-</SUP> to NO<SUB>2</SUB><SUP>-</SUP> is performed at 60&deg;C. Method: OD540nm. Samples: Biological. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.25 U/L.

25.   EnzyChrom™ Oxaloacetate Assay Kit     EOAA-100       100       £409.00             -20 °C   For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Linear detection range: 7 to 400 &micro;M oxaloacetate for colorimetric assays and 1 to 40 &micro;M for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 1 &micro;M.

26.   EnzyChrom™ Acetate Assay Kit EOAC-100             100       £439.00             -20 °C   For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.

27.   EnzyChrom™ Oxalate Assay Kit EOXA-100             100       £579.00             -20 °C   For quantitative determination of oxalate (oxalic acid) in urine and other samples. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 &micro;M oxalate. Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor&#8217;s assay kits. High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day. Method: OD595nm. Samples: urine, animal and plant tissue samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 &micro;M.

28.   EnzyChrom™ Phenylalanine Assay Kit    EPHE-100       100       £399.00             -20 °C   For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 2 - 300 &micro;M L-phenylalanine. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/595nm. Samples: Serum, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 &micro;M.

29.   EnzyChrom™ Phospholipd Assay Kit       EPLP-100       100       £459.00             -20 °C   For quantitative determination of phospholipid and evaluation of drug effects on its metabolism. Key Features: Sensitive. Use 20 &micro;L samples. Linear detection range: colorimetric assay 3 - 200 &micro;M, fluorimetric assay 0.6 - 20 &micro;M phospholipid. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 3, 0.6 &micro;M.

30.   EnzyChrom™ Phospholipase D Assay Kit             EPPD-100          100       £369.00             -20 °C   For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features: Sensitive. Use 10 &micro;L samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L.

31.   EnzyChrom™ Pyruvate Kinase Assay Kit EPRK-100       100       £509.00             -20 °C   For quantitative determination of pyruvate kinase (PK) activity and evaluation of drug effects on PK activity. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 50 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays run at 25&deg;C for 30 min. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/590nm. Samples: Plasma, serum, cell and tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

32.   EnzyChrom™ Pyruvate Assay Kit             EPYR-100       100       £409.00             -20 °C   For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate: 2 to 500 &micro;M (17 &micro;g/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 &micro;M for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 &micro;M.

33.   EnzyFluo™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit          ERBT-100             100       £509.00             -20 °C   For determination of RB(T821/826) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

34.   EnzyFluo™ Human/Mouse RPS6(S235/236) Phosphorylation ELISA  ERPS6-100         100             £509.00             -20 °C   For determination of RPS6(S235/236) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

35.   EnzyChrom™ Sorbitol Assay Kit ESBT-100             100       £479.00             -20 °C   For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 5 to 1000 &micro;M D-sorbitol. Convenient and high-throughput. Room temperature assay. No 37&deg;C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. blood), food, beverage and agriculture samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 &micro;M.

36.   EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit          ESHP2-100             100       £509.00             -20 °C   For determination of SHP2(Y542) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

37.   EnzyChrom™ Sialic Acid Assay Kit           ESLA-100       100       £509.00             -20 °C   For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 &micro;M for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 &micro;M.

38.   EnzyChrom™ Succinate Assay Kit            ESNT-100       100       £629.00             -20 °C   For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. Key Features: Fast and sensitive. Use 20 &micro;L sample. Linear detection range 10 to 400 &micro;M for colorimetric assays and 2 to 40 &micro;M for fluorimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: succinate in food, beverage, agricultural products, and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: Colorimetric assay: 10 &micro;M; Fluorimetric assay: 2 &micro;M.

39.   EnzyChrom™ Superoxide Dismutase Assay Kit             ESOD-100          100       £369.00             -20 °C   For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects. Key Features: Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD440nm. Samples: Blood, cell, tissue etc. Species: All. Procedure: Assay takes 1 hr. Kit size: 100 tests. Detection limit: 0.05 U/mL.

40.   EnzyChrom™ Triglyceride Assay Kit        ETGA-200       200       £439.00             -20 °C   For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range 0.01 to 1.0 mmol/L (0.88 to 88.5 mg/dL) triglyceride. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.01 mmol/L (0.88 mg/dL).

41.   EnzyFluo™ Tryptophan Assay Kit            ETRP-100       100       £579.00             -20 °C   For quantitative determination of tryptophan and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 &micro;L sample. Linear detection range from 10&micro;M to 400 &micro;M tryptophan in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 10 &micro;M.

42.   EnzyChrom™ Urea Assay Kit III EUR3-100             100       £329.00             -20 °C   For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 50 to 1000 &micro;M urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 &micro;M.

43.   EnzyChrom™ Xanthine Assay Kit             EXAN-100       100       £349.00             -20 °C   For quantitative determination of xanthine and drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate for 30 minute incubation: 0.01 to 2.5 mM xanthine for colorimetric assays and 3 to 250 &micro;M for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell lysate, serum, and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: FL 3 &micro;M; OD 10 &micro;M.

44.   EnzyChrom™ Xanthine Oxidase Assay Kit             EXOX-100          100       £359.00             -20 °C   For quantitative determination of xanthine oxidase enzyme activity and evaluation of its modulators. Key Features: Sensitive and accurate. Use as little as 10 &micro;L samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm. Samples: Cell lysate, serum, and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: FL 0.01 U/L; OD 0.03 U/L.

45.   QuantiFluo™ Acid Phosphatase Assay Kit             FACP-100           100       £159.00             -20 °C   For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 &micro;L sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.

46.   Urinary Indican Test Kit I1000N 20             £299.00             RT         An accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. For research use only, not intended for diagnostic use. The Indican Reagent is corrosive. It is recommended to perform the test in a chemical fume hood. Wear gloves, goggles and protective clothing. Key Features: Convenient. Only need to pipette 2 mL urine into the ready reagent vial, mix and read the indican level from a color chart. Fast: 15 min. Method: Obermeyer (Improved). Samples: Urine. Species: Human. Procedure: Assay takes 15 min. Kit size: 20 tests.

47.   QuantiChrom™ Acetylcholinesterase Inhibitor Assay Kit            IACE-100            100             £379.00             -20 °C   For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators. Key Features: Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Acetylcholinesterase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

48.   QuantiChrom™ Arginase Inhibitor Screening Kit         IARG-100           100       £269.00             -20 °C   For evaluation and high-throughput screen (HTS) of arginase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous &ldquo;mix-incubate-measure&rdquo; type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 2 hours and no 37&deg;C heater is needed. Method: OD430nm. Samples: Arginase. Species: All. Procedure: Assay takes 1 hour 45 min. Kit size: 100 tests.

49.   QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit              IGPDH-100        100       £419.00             -20 °C   For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous &ldquo;mix-incubate-measure&rdquo; type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37&deg;C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

79.   Meta-Phosphoric Acid   MPA-2G             100             £99.00  -20 °C   meta-Phosphoric Acid is a chemical reagent used for sample deproteination with the <a href="Glutathione-Assay-Kit-(EGTT-100).html" target="_blank">GSH/GSSG Assay Kit</a> (cat#: EGTT-100).

80.   Parallel Artificial Membrane Permeability Assay (PAMPA) Kit         PAMPA-096      96             £429.00             -20 °C   For quantitative determination of gastrointestinal (GI) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Species: All. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.

81.   Phosphatase Inhibitor (Sodium Orthovanadate)              PHIVA-1mL        NA             £65.00  -20 °C   Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems' vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM.

82.   Parallel Artificial Membrane Permeability Assay-BBB Kit   PMBBB-096       96             £429.00             -20 °C   For quantitative determination of blood brain barrier (BBB) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.

83.   Parallel Artificial Membrane Permeability Assay-Skin Kit   PMSKN-096       96             £429.00             -20 °C   For quantitative determination of skin membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical Compounds. Procedure: Assay takes 20 hrs, hands-on time 1 hr. Kit size: 96 tests.

84.   Malachite Green Phosphate Assay Kit             POMG-25H        2500     £259.00             4 °C       For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 &micro;M and 40 &micro;M phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm (malachite green). Samples: Biological, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 2500 tests. Detection limit: 0.02 &micro;M.

85.   PiBlue™ Phosphate Assay Kit     POPB-500             500       £239.00             4°C        For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 &micro;M and 50 &micro;M phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 &micro;M.

86.   pNPP Phosphatase Assay Kit      POPN-500             500       £249.00             4 °C       For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng.

87.   QuantiChrom™ Protein Assay Kit            QCPR-500       500       £249.00             -20 °C   For quantitative determination of total protein. Key Features: Sensitive and accurate. Use 10 mL samples. Detection range 0.06 - 1.0 mg /mL protein in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay. Versatility: assays can be executed in 96-well plate or cuvet. Method: OD595nm (Bradford). Samples: Biological, food, agriculture etc. Species: All. Procedure: Assay takes 2 min. Kit size: 500 tests. Detection limit: 60 &micro;g/mL.

88.   QuantiFluo™ ALP Detection Reagent             QFALP-6mL        600       £129.00             -20 °C   Stabilized 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (360/450 nm) when dephosphorylated by ALP. The fluorescent product can also be visualized under a UV light. Key Features: Stable single reagent. Highly sensitive fluorescence detection. Can be visualized under a UV light. Method: FL360/450nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 600 tests.

89.   QuantiFluo™ Alkaline Phosphatase Assay Kit             QFAP-100          100       £229.00             -20 °C   For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Key Features: High sensitivity and wide linear range. Use 10 &micro;L sample. Detection limit of 0.02 U/L (20 min reaction). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: FL360/450nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 U/L.

90.   QuantiFluo™ DNA Assay Kit       QFDN-250             250       £359.00             4 °C       For quantitative determination of DNA. Key Features: Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day. Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay. Method: FL350/450nm. Samples: Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 100 ng/mL.

91.   QuantiFluo™ Diamine Oxidase Assay Kit             QFDO-100         100       £499.00             -20 °C   For quantitative determination of diamine oxidase and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 10 &micro;L sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25&deg;C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37&deg;C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Serum, Plasma. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

92.   QuantiFluo™ HRP Detection Reagent             QFHRP-25mL     500       £249.00             -20 °C   Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/585nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm). Key Features: For both absorbance or fluorescence detection. Highly sensitive. Convenient and fast. Method: OD570nm, FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests.

93.   QuantiFluo™ Protein Assay Kit  QFPR-200             200       £159.00             -20 °C   For quantitative determination of total protein. Key Features: Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 &micro;g/mL BSA. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological. Species: All. Procedure: Assay takes 10 min. Kit size: 200 tests. Detection limit: 50 ng/mL.

94.   QuantiQuik™ Albumin Quick Test Strips             QQALBM10       10         £66.00  RT             For rapid, direct determination of albumin concentrations in biological samples (e.g. serum, plasma, urine, etc). Key Features: Fast and sensitive. Use of 10 &micro;L sample. Semi-quantitative measurement between 0-0.4 g/dL or 0-60 &micro;M (undiluted) Albumin. Convenient. No sophisticated laboratory equipment required. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

95.   QuantiQuik™ D-Lactic Acid Quick Test Strips             QQDLAC10        10         £66.00  RT             For rapid, direct determination of D-lactic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 &micro;L sample. Semi-quantitative measurement between 0-180 mg/L (undiluted) D-lactic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

96.   QuantiQuik™ Glucose Quick Test Strips             QQGLUC10        10         £66.00  RT             For rapid, direct determination of glucose concentrations in food and beverage samples as well as biological (e.g. urine, blood, etc.) samples. Key Features: Fast and sensitive. Use of 20 or 100 &micro;L sample. Semi-quantitative measurement between 0-720 mg/L (undiluted) Glucose. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, juice, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

97.   QuantiQuik™ L-Glutamate Quick Test Strips             QQGLUT10        10         £66.00  RT             For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 &micro;L sample. Semi-quantitative measurement between 0-300 mg/L (undiluted) L-Glutamate. Convenient. No extra equipment required. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Soups, sauces, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

98.   QuantiQuik™ Histamine Quick Test Strips             QQHIST10          10         £66.00  RT             For rapid, direct determination of Histamine concentrations in fish, wine, and other samples. Key Features: Fast and sensitive. Use of 500 mg of fish or 250 &micro;L of sample. Semi-quantitative measurement between 0-200 ppm (undiluted) Histamine. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Seafood, wine, juice, milk and dairy products, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

99.   QuantiQuik™ L-Lactic Acid Quick Test Strips             QQLLAC10         10         £66.00  RT             For rapid, direct determination of L-lactic acid concentrations in food and beverage samples as well as biological samples. Semi-quantitative measurement between 0-360 mg/L (undiluted) L-lactic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

100.                       QuantiQuik™ Malic Acid Quick Test Strips    QQMALA10       10         £66.00  RT             For rapid, direct determination of malic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 &micro;L sample. Semi-quantitative measurement between 0-500 mg/L (undiluted) malic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

101.                       QuantiQuik™ Oxalate Quick Test Strips    QQOXLT10        10         £66.00  RT             For rapid, direct determination of oxalate concentrations in biological samples. For Research Use Only (not for medical decisions). Key Features: Fast and sensitive. Use of 10 &micro;L sample. Semi-quantitative measurement between 0-1.5 mg/dL or 0-170 &micro;M (undiluted) Oxalate. Convenient. No expensive plate or cuvette readers required. Sample treatment and assay can be performed in under 10 minutes. Method: Visual. Samples: Serum, plasma, urine, fruits or vegetables, etc. Species: All. Procedure: Assay takes approximately 10 min. Kit size: 10 tests.

102.                       QuantiQuik™ Pyruvate Quick Test Strips    QQPYR10           10         £66.00  RT             For rapid, direct determination of pyruvate concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 50, 100, or 400 &micro;L sample. Semi-quantitative measurement between 0-131 mg/L (undiluted) Pyruvate. Convenient. No expensive plate or cuvette readers needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, biological samples, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

103.                       QuantiQuik™ Urea (BUN) Quick Test Strips    QQUREA10        10         £66.00  RT             For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 &micro;L sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

104.                       QuantiChrom™ Total Protein Assay Kit             QTPR-01K          1000     £219.00             4°C        For quantitative determination of total protein. Key Features: Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein. Robust and Reliable. Substances in urine have minimal interference (< 2%). Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD600nm (Pyrogallol Red). Samples: Urine, cerebrospinal fluid etc. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 5 mg/dL.

105.                       QuantiChrom™ Total Protein Assay Kit             QTPR-100          100       £99.00  4°C             For quantitative determination of total protein. Key Features: Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein. Robust and Reliable. Substances in urine have minimal interference (< 2%). Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD600nm (Pyrogallol Red). Samples: Urine, cerebrospinal fluid etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 5 mg/dL.

106.                       SuperLight™ Dual Luciferase Reporter Gene Assay Kit  SLDL-100           100             £189.00             -20 °C             Bioluminescent reagent system for rapid quantitation of <i>firelfy</i> and <i>Ranilla luciferase</i> reporter gene expression in transfected cells. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems. Fast and convenient. Three step assay allows detection of dual luciferase levels within 20 minutes. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 fg luciferase.

107.                       SuperLight™ Luciferase Reporter Gene Assay Kit  SLLU-01K           1000             £459.00             -20 °C   Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems. Fast and convenient. Homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection. Robust and amenable to HTS. Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 2 min. Kit size: 1000 tests. Detection limit: 2 fg luciferase.

108.                       SuperLight™ Luciferase Reporter Gene Assay Kit  SLLU-200           200             £169.00             -20 °C   Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems. Fast and convenient. Homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection. Robust and amenable to HTS. Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 2 min. Kit size: 1000 tests. Detection limit: 2 fg luciferase.

109.                       SuperLight™ Luciferase Reporter Gene Assay Kit  SLLU-500           500             £299.00             -20 °C   Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems. Fast and convenient. Homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection. Robust and amenable to HTS. Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 2 min. Kit size: 1000 tests. Detection limit: 2 fg luciferase.

 

Accessories       

 

                                                                                                                                       i.       

2.       Empty Tubes (10)          2MLET-10          10             £17.00  RT         Empty tubes used for preparation of urine samples for use with the Oxalate Quick Test Strips. Quantity: 10 empty 2mL tubes.

3.       Carbon Powder CARB-10            10             £17.00  RT         Carbon powder used for preparation of urine samples for use with the Oxalate Quick Test Strips. Quantity: 10 tubes of 50 mg carbon powder.

4.       Mini Centrifuge CTFG     N/A       £39.00  RT             Small centrifuge used for preparation of fish and wine samples for use with the Histamine Quick Test Strips. Quantity: 1.

5.       0.5M EDTA        EDTA-150          150 µL             £15.00  RT         0.5M EDTA used for preparation of urine samples for use with the Oxalate Quick Test Strips. Quantity: 150 &micro;L 0.5M EDTA.

6.       PVPP Powder    PVPP-10             10             £15.00  RT         Polyvinylpyrrolidone (PVPP) used for preparation of wine samples for use with the Histamine Quick Test Strips.

7.       Microtiter Plates (P96BCC)        P96BCC             96Well £19.00  RT         Black Clear Bottom 96-Well Plate, Sterile, with Lid, for Cell-based Fluorometric Assays.

8.       Microtiter Plates (P96CCC)        P96CCC             96Well £19.00  RT         Clear Flat-Bottom 96-Well Plate, Sterile, with Lid, for Cell-based Colorimetric Assays.

9.       Microtiter Plates (P96FL)            P96FL             96Well £10.00  RT         Black Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for Fluorometric Assays.

10.   Microtiter Plates (P96OD)          P96OD             96Well £9.00    RT         Clear Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for OD Assays at >350nm.

11.   Microtiter Plates (P96UV)          P96UV             96Well £23.00  RT         Clear Flat-Bottom 96-Well UV Plate, Nonsterile, with Lid, for OD Assays at >230nm.

12.   Microtiter Plates (P96WT)         P96WT             96Well £9.00    RT         White Opaque Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for Luminescence Assays.

13.   100µL Transfer Pipettes             TP100   N/A             £15.00  RT         For pipetting 100&micro;L samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.

14.   1000µL Transfer Pipettes           TP1000 N/A             £15.00  RT         For pipetting 1000&micro;L samples. To be used with the Histamine Quick Test Strips. Key Features:  Single use, disposable, easy-to-use. Quantity: 10 pipettes.

15.   20µL Transfer Pipettes  TP20     N/A             £15.00  RT         For pipetting 20&micro;L samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features:  Single use, disposable, easy-to-use. Quantity: 10 pipettes.

16.   200µL Transfer Pipettes             TP200   N/A             £15.00  RT         For pipetting 200&micro;L samples. To be used with the Histamine Quick Test Strips. Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

17.   300µL Transfer Pipettes             TP300   N/A             £15.00  RT         For pipetting 300&micro;L samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

18.   400µL Transfer Pipettes             TP400   N/A             £15.00  RT         For pipetting 400&micro;L samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

19.   50µL Transfer Pipettes  TP50     N/A             £15.00  RT         For pipetting 50&micro;L samples. To be used with the Pyruvate Quick Test Strips. Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

20.   Deionized Water            DH2O-10           10             £15.00  RT         Deionized water used for preparation of samples for use with the Urea Quick Test Strips. Quantity: 10 mL.

21.   Eppendorf Tubes (50)   EPP-50  50             £15.00  RT         To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 Eppendorf tubes.

22.   PCR Tubes (50) PCR-50 50         £15.00  RT             To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

 

Distributors

Europe Gentaur Bv    

EuropeGentaur Bv     

 

Belgium Gentaur BV,        TEL +32 2 265 09 20 / Voortstraat 49, 1910 Kampenhout                                 BE0473327336

Netherlands Gentaur Bv  TEL +31 20 808 0893  / Kuiper 1, 5521 DG Eersel The Netherlands                 NL850396268B01

Germany Gentaur GmbH TEL +49 0241 93688188 /             Marienbongard 20, 52062 Aachen              DE815175831

SpainGentaur España      TEL + 34 0911 87 65 58  /             Avenida de Andalucía, 27, 29006 Málaga   BE0473327336

FranceGentaur France SARL TEL +33 01 43 25 01 50 /             9 Rue Lagrange, 75005 Paris                    FR63484237888

ItalyGentaur Italia SRL         TEL +39 0350086186 / Piazza Giacomo Matteotti n.6, 24122 Bergamo    BG IT03841300167

PolandGentaur Sp. z o.o. TEL +48 058 710 33 44 / lok.2, Grunwaldzka 88A, 81-771 Sopot                    PL2040002710

BulgariaGentaur EOOD    TEL +359 089 745 5388 / Ul iSkar 53, 1191 Kokalyane                                      BG201358931

 

UK Gentaur UK Ltd.    TEL +44 020 3393 8531 / Station Close, Unicorn House, Potters Bar EN6 1TL    GB111298832

USA Genprice Inc.       TEL +1 4087800908 / 6017 Snell Avenue, San Jose, CA 95123

 

Worldwide online orders Maxanim